Recombinant Human N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) (Active), Homo sapiens (Human), E.coli, N-terminal GST-tagged, 52.7 kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) (Active)

Recombinant Human N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) (Active) — Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Purity >85%.

SKU: BCACP-000048 Categories: ,
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Product SKUBCACP-000048
Product DescriptionRecombinant Human N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) protein is expressed from E.coli with N-terminal GST-tagged. It contains 2-223aa. [Accession | Q9BV86].
Uniprot No.Q9BV86
Gene NamesNTMT1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-223aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal GST-tagged
Molecular weight52.7 kDa
ActivityMeasured by its binding ability in a functional ELISA.Immobilized Human CD38 at 2 μg/mL can bind Anti-CD38 recombinant antibody. The EC50 is 20.07-28.73 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesAlpha N-terminal protein methyltransferase 1A;Methyltransferase-like protein 11A;N-terminal RCC1 methyltransferase;X-Pro-Lys N-terminal protein methyltransferase 1A;NTM1A

Function

Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the [Ala/Gly/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by NTMT2-mediated monomethylation.

Biological Context

Subcellular Location: Nucleus

Product Specifications

Recombinant Human N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) (Active) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 2-223aa, with N-terminal GST-tagged tag, molecular weight 52.7 kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA.Immobilized Human CD38 at 2 μg/mL can bind Anti-CD38 recombinant antibody. The EC50 is 20.07-28.73 ng/mL.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the [Ala/Gly/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by NTMT2-mediated monomethylation (PubMed:24090352). Catalyzes the trimethylation of the N-terminal Gly in CENPA (after removal of Met-1). Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1

Subcellular Location

Nucleus

Gene: NTMT1  |  Organism: Homo sapiens  |  Synonyms: Alpha N-terminal protein methyltransferase 1A; Methyltransferase-like protein 11A; N-terminal RCC1 methyltransferase; X-Pro-Lys N-terminal protein methyltransferase 1A
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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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