Recombinant Human N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) (Active), Homo sapiens (Human), E.coli, N-terminal GST-tagged, 52.7 kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) (Active)

Recombinant Human N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) (Active) — Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Purity >85%.

SKU: BCACP-000048 Categories: ,
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Product SKUBCACP-000048
Product DescriptionRecombinant Human N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) protein is expressed from E.coli with N-terminal GST-tagged. It contains 2-223aa. [Accession | Q9BV86].
Uniprot No.Q9BV86
Gene NamesNTMT1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-223aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal GST-tagged
Molecular weight52.7 kDa
ActivityMeasured by its binding ability in a functional ELISA.Immobilized Human CD38 at 2 μg/mL can bind Anti-CD38 recombinant antibody. The EC50 is 20.07-28.73 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesAlpha N-terminal protein methyltransferase 1A;Methyltransferase-like protein 11A;N-terminal RCC1 methyltransferase;X-Pro-Lys N-terminal protein methyltransferase 1A;NTM1A

Function

Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the [Ala/Gly/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by NTMT2-mediated monomethylation.

Biological Context

Subcellular Location: Nucleus

Product Specifications

Recombinant Human N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) (Active) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 2-223aa, with N-terminal GST-tagged tag, molecular weight 52.7 kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the [Ala/Gly/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by NTMT2-mediated monomethylation (PubMed:24090352). Catalyzes the trimethylation of the N-terminal Gly in CENPA (after removal of Met-1). Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1

Subcellular Location

Nucleus

Gene: NTMT1  |  Organism: Homo sapiens  |  Synonyms: Alpha N-terminal protein methyltransferase 1A; Methyltransferase-like protein 11A; N-terminal RCC1 methyltransferase; X-Pro-Lys N-terminal protein methyltransferase 1A
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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