Recombinant Human Repulsive guidance molecule A (RGMA), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 42.9 kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Human Repulsive guidance molecule A (RGMA), partial (Active)

Recombinant Human Repulsive guidance molecule A (RGMA), partial (Active) — Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Purity >95%.

SKU: BCACP-000541 Categories: , ,
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Product SKUBCACP-000541
Product DescriptionRecombinant Human Repulsive guidance molecule A (RGMA) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 48-422aa. [Accession | Q96B86].
Uniprot No.Q96B86
Gene NamesRGMA
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region48-422aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight42.9 kDa
ActivityMeasured by its binding ability in a functional ELISA.Immobilized Macaca fascicularis IL23A&IL12B at 2 μg/mL can bind anti-IL23A&IL12B recombinant antibody.The EC50 is 0.7723-0.9250 ng/mL.;IL23A&IL12B Recombinant Monoclonal Antibody captured on Protein A Chip can bind Macaca fascicularis IL23A&IL12B with an affinity constant of 0.27 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesRepulsive guidance molecule A; RGM domain family member A

Function

Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition.

Biological Context

Subcellular Location: Cell membrane (Lipid-anchor, GPI-anchor)

Product Specifications

Recombinant Human Repulsive guidance molecule A (RGMA), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 48-422aa, with C-terminal 10xHis-tagged tag, molecular weight 42.9 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8

Subcellular Location

Cell membrane (Lipid-anchor, GPI-anchor)

Subunit

Interacts with NEO1, BMP2 and BMP4

Gene: RGMA  |  Organism: Homo sapiens  |  Synonyms: RGM domain family member A
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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