Function
Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex.
Product Specifications
Recombinant Human Roundabout homolog 1 (ROBO1), partial (Active) is a recombinant protein, expressed in Mammalian cell, covering amino acids 26-897aa, with C-terminal 10xHis-tagged tag, molecular weight 97.0 kDa, purity Greater than 90% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.
Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.
Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human MICA at 2 μg/mL can bind Anti-MICA/B recombinant antibody . The EC50 is 1.818-2.1050 ng/mL.;MICA/B Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Human MICA with an affinity constant of 1.13 nM as detected by MetaSPR Assay (WeSPRTM 200).
Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.
