Recombinant Human SHC-transforming protein 1 (SHC1), partial, Biotinylated

Function

Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis. Involved in the oncostatin-M-mediated signaling pathway through type II OSM receptor complex (heterodimers composed of OSMR and IL6ST) where it contributes to activation of the Ras/Raf/MAPK pathway through JAK1-dependent recruitment to OSMR subunit of OSMR complex and SHC1 association with GRB2 (PubMed:11016927)

Subcellular Location

Mitochondrion

Tissue Specificity

Widely expressed. Expressed in neural stem cells but absent in mature neurons

Subunit

(Microbial infection) Interacts with herpes simplex virus 1 UL46

• Pelicci G. et al. A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction. — Cell (1992)
• Migliaccio E. et al. Opposite effects of the p52shc/p46shc and p66shc splicing isoforms on the EGF receptor-MAP kinase-fos signalling pathway. — EMBO J. (1997)
• Harun R.B. et al. Characterization of human SHC p66 cDNA and its processed pseudogene mapping to Xq12-q13.1. — Genomics (1997)
• Ota T. et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. — Nat. Genet. (2004)
• Goshima N. et al. Human protein factory for converting the transcriptome into an in vitro-expressed proteome. — Nat. Methods (2008)
• Gregory S.G. et al. The DNA sequence and biological annotation of human chromosome 1. — Nature (2006)
• Mural R.J. et al. PubMed: CI-5GBD0VIIJ7C63 (SEP-)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)