Recombinant Human T-cell surface glycoprotein CD5 (CD5), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 40.0 kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human T-cell surface glycoprotein CD5 (CD5), partial (Active)

Recombinant Human T-cell surface glycoprotein CD5 (CD5), partial (Active) — Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Purity >90%.

SKU: BCACP-000577 Categories: ,
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Product SKUBCACP-000577
Product DescriptionRecombinant Human T-cell surface glycoprotein CD5 (CD5) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 25-372aa. [Accession | P06127].
Uniprot No.P06127
Gene NamesCD5
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region25-372aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight40.0 kDa
ActivityMeasured by its binding ability in a functional ELISA.Immobilized Human KIT at 2 μg/mL can bind Human KITLG. The EC50 is 0.6921-0.8551 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesLymphocyte antigen T1/Leu-1; CD5; LEU1

Function

Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Human T-cell surface glycoprotein CD5 (CD5), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 25-372aa, with C-terminal 10xHis-tagged tag, molecular weight 40.0 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA.Immobilized Human KIT at 2 μg/mL can bind Human KITLG. The EC50 is 0.6921-0.8551 ng/mL.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL (PubMed:1384049, PubMed:1385158). Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation (PubMed:23376399). Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production (PubMed:27499044)

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Subunit

Interacts with CD72/LYB-2 (PubMed:1711157). Interacts with PTPN6/SHP-1 (By similarity). Interacts with CBL (PubMed:23376399). Interacts with CD5L (By similarity). Interacts with CD3Z/CD247 (PubMed:1384049, PubMed:1385158)

Gene: CD5  |  Organism: Homo sapiens  |  Synonyms: Lymphocyte antigen T1/Leu-1
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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