Recombinant Human Tapasin (TAPBP), partial, Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 49.4kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Tapasin (TAPBP), partial

Recombinant Human Tapasin (TAPBP), partial — recombinant protein from Homo sapiens (Human), expressed in E.coli. Purity >85%.

SKU: BCRECP-001735 Category:
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Product Specifications

Product SkuBCRECP-001735
Product DescriptionRecombinant Human Tapasin (TAPBP) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 21-414aa. [Accession | O15533].
Uniprot No.O15533
Gene NamesTAPBP
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region21-414aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight49.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).

Biological Context

Subcellular Location: Endoplasmic reticulum membrane (Single-pass type I membrane protein)
Tissue Specificity: Neutrophils, mostly in fully differentiated cells
Disease Association: MHC class I deficiency 3 (MHC1D3) : An autosomal recessive disorder characterized by glomerulonephritis and markedly reduced cell surface expression of class I HLA antigens. Additional features are herpes zoster infection and polyps of the stomach and colon. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Tapasin (TAPBP), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 21-414aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 49.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading)

Subcellular Location

Endoplasmic reticulum membrane (Single-pass type I membrane protein)

Disease Association

MHC class I deficiency 3 (MHC1D3) : An autosomal recessive disorder characterized by glomerulonephritis and markedly reduced cell surface expression of class I HLA antigens. Additional features are herpes zoster infection and polyps of the stomach and colon. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Neutrophils, mostly in fully differentiated cells

Subunit

Heterodimer with PDIA3; disulfide-linked. Obligatory mediator for the interaction between newly assembled MHC class I molecules, calreticulin, PDIA3 and TAP. Up to 4 MHC class I/tapasin complexes bind to 1 TAP (PubMed:19119025, PubMed:21263072, PubMed:26611325). Interacts with HLA-G-B2M complex; this interaction is required for loading of high affinity peptides (PubMed:21263072). On its own or as part of MHC class I peptide loading complex, interacts with ligand-free MR1 or MR1-B2M complex, providing for stable MR1 pools ready for metabolite antigen processing (PubMed:32958637)

Gene: TAPBP  |  Organism: Homo sapiens  |  Synonyms: NGS-17; TAP-associated protein; TAP-binding protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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