Recombinant Human Tetraspanin-8 (TSPAN8)-VLPs (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged(This tag can be tested only under denaturing conditions), 27.4 kDa
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Recombinant Human Tetraspanin-8 (TSPAN8)-VLPs (Active)

Recombinant Human Tetraspanin-8 (TSPAN8)-VLPs (Active) — Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling.

SKU: BCACP-000328 Categories: ,
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Product SKUBCACP-000328
Product DescriptionRecombinant Human Tetraspanin-8 (TSPAN8)-VLPs protein is expressed from Mammalian cell with C-terminal 10xHis-tagged(This tag can be tested only under denaturing conditions). It contains 1-237aa. [Accession | P19075].
Uniprot No.P19075
Gene NamesTSPAN8
Expression SystemMammalian cell
Expression Region1-237aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged(This tag can be tested only under denaturing conditions)
Molecular weight27.4 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis PTK7 at 2 μg/mL can bind Anti-PTK7 recombinant antibody . The EC50 is 1.399 - 1.666 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesTransmembrane 4 superfamily member 3; Tumor-associated antigen CO-029

Function

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, migration and protein trafficking. Promotes ADAM17-mediated TNF processing through recruitment of ADAM17 to tetraspanin-enriched micro-domains (TEMs).

Product Specifications

Recombinant Human Tetraspanin-8 (TSPAN8)-VLPs (Active) is a recombinant protein, expressed in Mammalian cell, covering amino acids 1-237aa, with C-terminal 10xHis-tagged(This tag can be tested only under denaturing conditions) tag, molecular weight 27.4 kDa. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed:27180357, PubMed:36078095). Participates thereby in diverse biological functions such as cell signal transduction, migration and protein trafficking (PubMed:25761241). Promotes ADAM17-mediated TNF processing through recruitment of ADAM17 to tetraspanin-enriched micro-domains (TEMs) (PubMed:36078095). Forms a complex with RICTOR and integrin alpha3/ITGA3 to mediate mTORC2 activation and AKT1 phosphorylation leading to cell migration (PubMed:25761241). Reduces apoptosis and autophagy induced by high glucose levels through forming a complex with mTOR and RICTOR (PubMed:35904232). Contributes to the maintenance of intestinal epithelial barrier and plays a role in the regulation of intestine inflammation by switching interferon gamma receptor 1/IFNGR1 from clathrin-dependent to lipid raft-dependent endocytosis route to limit STAT1 activation magnitude and duration (PubMed:37204469). Acts as a modulator of the endothelin axis by associating with endothelin converting enzyme ECE1 and regulating its activity of conversion of the endothelin-1 precursor to endothelin (PubMed:37835445)

Gene: TSPAN8  |  Organism: Homo sapiens

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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