Recombinant Human Tumor necrosis factor ligand superfamily member 14 (TNFSF14), partial (Active), Homo sapiens (Human), Mammalian cell, N-terminal hFc-Myc-tagged, 46.7 kDa, purity >85% as determined by SDS-PAGE.
,

Recombinant Human Tumor necrosis factor ligand superfamily member 14 (TNFSF14), partial (Active)

Recombinant Human Tumor necrosis factor ligand superfamily member 14 (TNFSF14), partial (Active) — Cytokine that binds to TNFRSF3/LTB and TNFRSF14/HVEM and plays major roles in immune system regulation. Purity >85%.

SKU: BCACP-000087 Categories: ,
Online Inquiry →
Product SKUBCACP-000087
Product DescriptionRecombinant Human Tumor necrosis factor ligand superfamily member 14 (TNFSF14) protein is expressed from Mammalian cell with N-terminal hFc-Myc-tagged. It contains 74-240aa. [Accession | O43557].
Uniprot No.O43557
Gene NamesTNFSF14
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region74-240aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal hFc-Myc-tagged
Molecular weight46.7 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (N501Y) at 2 μg/ml can bind human ACE2 , the EC50 is 2.492-4.401 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesTNFSF14; HVEML; LIGHT; UNQ391/PRO726; Tumor necrosis factor ligand superfamily member 14; Herpes virus entry mediator ligand; HVEM-L; Herpesvirus entry mediator ligand; CD antigen CD258

Function

Cytokine that binds to TNFRSF3/LTB and TNFRSF14/HVEM and plays major roles in immune system regulation. Upon binding to TNFRSF14/HVEM, delivers costimulatory signals to T cells, leading to T cell proliferation and IFNG production. Binds to the decoy receptor TNFRSF6B thereby disrupts the activation signal initiated by TNFSF14, resulting in reduced T-cell activation.

Biological Context

Subcellular Location: Cytoplasm
Tissue Specificity: Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines

Product Specifications

Recombinant Human Tumor necrosis factor ligand superfamily member 14 (TNFSF14), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 74-240aa, with N-terminal hFc-Myc-tagged tag, molecular weight 46.7 kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Cytokine that binds to TNFRSF3/LTB and TNFRSF14/HVEM and plays major roles in immune system regulation (PubMed:10754304, PubMed:9462508). Upon binding to TNFRSF14/HVEM, delivers costimulatory signals to T cells, leading to T cell proliferation and IFNG production (PubMed:10754304). Binds to the decoy receptor TNFRSF6B thereby disrupts the activation signal initiated by TNFSF14, resulting in reduced T-cell activation (PubMed:25087510)

Subcellular Location

Cytoplasm

Tissue Specificity

Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines

Subunit

Homotrimer. TNFSF14 binds to TNFRSF6B with 3:3 stoichiometry (PubMed:25087510). Interacts with TNFRSF14

Gene: TNFSF14  |  Organism: Homo sapiens  |  Synonyms: Herpes virus entry mediator ligand
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top