Recombinant Human Tumor necrosis factor ligand superfamily member 15 (TNFSF15) (Active), Homo sapiens (Human), E.coli, Tag-Free, 21.86 kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Human Tumor necrosis factor ligand superfamily member 15 (TNFSF15) (Active)

Recombinant Human Tumor necrosis factor ligand superfamily member 15 (TNFSF15) (Active) — Cytokine that signals through TNFRSF25 receptor. Purity >95%.

SKU: BCACP-001251 Categories: ,
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Product SKUBCACP-001251
Product DescriptionRecombinant Human Tumor necrosis factor ligand superfamily member 15 (TNFSF15) protein is expressed from E.coli with Tag-Free. It contains 1-192aa. [Accession | O95150].
Uniprot No.O95150
Gene NamesTNFSF15
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-192aa
SpeciesHomo sapiens (Human)
Tag InfoTag-Free
Molecular weight21.86 kDa
ActivityFully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesTNFSF15; TL1; VEGI; Tumor necrosis factor ligand superfamily member 15; TNF ligand-related molecule 1; Vascular endothelial cell growth inhibitor

Function

Cytokine that signals through TNFRSF25 receptor. Functions as a T-cell costimulator, that increases IL-2 responsiveness and secretion of proinflammatory cytokines which enhances T-cell activation, proliferation and the survival of activated T-cells. Inhibits vascular endothelial growth and angiogenesis (in vitro).

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Specifically expressed in endothelial cells. Detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon

Product Specifications

Recombinant Human Tumor necrosis factor ligand superfamily member 15 (TNFSF15) (Active) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-192aa, with Tag-Free tag, molecular weight 21.86 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Cytokine that signals through TNFRSF25 receptor (PubMed:11911831). Functions as a T-cell costimulator, that increases IL-2 responsiveness and secretion of proinflammatory cytokines which enhances T-cell activation, proliferation and the survival of activated T-cells (PubMed:10597252, PubMed:11911831). Inhibits vascular endothelial growth and angiogenesis (in vitro) (PubMed:11923219, PubMed:9872942). Binds to the decoy receptor TNFRSF6B, blocking the activation normally triggered by TNFSF15 and leading to decreased T-cell activation and reduced inflammation (PubMed:21300286)

Subcellular Location

Secreted

Tissue Specificity

Specifically expressed in endothelial cells. Detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon

Subunit

Homotrimer (PubMed:17935696). TNFSF15 binds TNFRSF6B with 3:3 stoichiometry (PubMed:21300286)

Gene: TNFSF15  |  Organism: Homo sapiens  |  Synonyms: TNF ligand-related molecule 1; Vascular endothelial cell growth inhibitor
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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