Recombinant Macaca mulatta Apelin receptor (APLNR)-VLPs (Active), Macaca mulatta (Rhesus macaque), Mammalian cell, C-terminal 10xHis-tagged, 44.1 kDa, purity >95% as determined by SEC-HPLC.
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Recombinant Macaca mulatta Apelin receptor (APLNR)-VLPs (Active)

Recombinant Macaca mulatta Apelin receptor (APLNR)-VLPs (Active) — recombinant protein from Macaca mulatta (Rhesus macaque), expressed in Mammalian cell. Purity >95%.

SKU: BCACP-000605 Categories: ,
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Product SKUBCACP-000605
Product DescriptionRecombinant Rhesus macaque Apelin receptor (APLNR)-VLPs protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 1-380aa. [Accession | O97666].
Uniprot No.O97666
Gene NamesAPLNR
PurityGreater than 95% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region1-380aa
SpeciesMacaca mulatta (Rhesus macaque)
Tag InfoC-terminal 10xHis-tagged
Molecular weight44.1 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis IL2RA at 2 μg/mL can bind Macaca fascicularis IL2 . The EC50 is 88.60-116.8 ng/mL.;Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis IL2RA at 2 μg/mL can bind Human IL2 . The EC50 is 0.8151-1.087 ng/mL.;Macaca fascicularis IL2 captured on Protein A Chip can bind Recombinant Macaca fascicularis IL2RA with an affinity constant of 31.3 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesApelin receptor; Angiotensin receptor-like 1; G-protein coupled receptor APJ

Function

(Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Macaca mulatta Apelin receptor (APLNR)-VLPs (Active) is a recombinant protein from Macaca mulatta (Rhesus macaque), expressed in Mammalian cell, covering amino acids 1-380aa, with C-terminal 10xHis-tagged tag, molecular weight 44.1 kDa, purity Greater than 95% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

(Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Subunit

Homodimer; dimerization inhibits APLNR-mediated G protein and beta-arrestin signaling pathways compared to monomeric APLNR

Gene: APLNR  |  Organism: Macaca mulatta  |  Synonyms: Angiotensin receptor-like 1; G protein-coupled receptor APJ

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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