Recombinant Mouse Cardiotrophin-1 protein (Ctf1), Mus musculus (Mouse), E.Coli, Tag-Free, 21.5 kDa, purity >>95% as determined by SDS­PAGE.

Recombinant Mouse Cardiotrophin-1 protein (Ctf1)

Recombinant Mouse Cardiotrophin-1 protein (Ctf1) — Functions as a cytokine that binds to the LIF receptor complex. Purity >95%.

SKU: BCACP-000876 Category:
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Product SKUBCACP-000876
Product DescriptionRecombinant Mouse Cardiotrophin-1 protein (Ctf1) protein is expressed from E.Coli with Tag-Free. It contains 1-203aa. [Accession | Q60753].
Uniprot No.Q60753
Gene NamesCtf1
Purity>95% as determined by SDS­PAGE.
Expression SystemE.Coli
Expression Region1-203aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight21.5 kDa
ActivityFully biologically active when compared to standard. The ED50 as determined by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells is less than 5.0 μg/ml, corresponding to a specific activity of >200 IU/m.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesCtf1Cardiotrophin-1; CT-1

Function

Functions as a cytokine that binds to the LIF receptor complex. The LIF receptor complex indeed consists of two signaling receptor subunits IL6ST/gp130 and LIFR that transduce the signal into the cell. Functionnally, acts as a potent cardiac survival factor, and promotes cardiomyocyte proliferation and hypertrophy.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels in testis and brain. No expression in spleen

Product Specifications

Recombinant Mouse Cardiotrophin-1 protein (Ctf1) is a recombinant protein from Mus musculus (Mouse), expressed in E.Coli, covering amino acids 1-203aa, with Tag-Free tag, molecular weight 21.5 kDa, purity >95% as determined by SDS­PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Functions as a cytokine that binds to the LIF receptor complex (PubMed:7738033, PubMed:9030543). The LIF receptor complex indeed consists of two signaling receptor subunits IL6ST/gp130 and LIFR that transduce the signal into the cell (PubMed:7738033, PubMed:9030543). Functionnally, acts as a potent cardiac survival factor, and promotes cardiomyocyte proliferation and hypertrophy (PubMed:7862649, PubMed:8625793, PubMed:9050750). Mechanistically, ligand binding to LIFR, induces heterodimerization with IL6ST/gp130 activating JAK tyrosine kinases (JAK1 or JAK2 and to a lesser extent TYK2) bound to their intracellular domains (PubMed:9030543). These kinases subsequently phosphorylate IL6ST/gp130 and LIFR (PubMed:9030543). The tyrosine phosphorylated signaling receptors serve in turn as docking sites for recruitment and activation of signal transducer and activator of transcription STAT3 (PubMed:9030543)

Subcellular Location

Secreted

Tissue Specificity

Highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels in testis and brain. No expression in spleen

Gene: Ctf1  |  Organism: Mus musculus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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