Recombinant Mouse Dehydrodolichyl diphosphate synthase complex subunit Nus1 (Nus1), partial, Mus musculus (Mouse), Mammalian cell, C-terminal hFc1-tagged, 46.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Mouse Dehydrodolichyl diphosphate synthase complex subunit Nus1 (Nus1), partial

Recombinant Mouse Dehydrodolichyl diphosphate synthase complex subunit Nus1 (Nus1), partial — With DHDDS, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Purity >90%.

SKU: BCRECP-003919 Categories: ,
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Product Specifications

Product SkuBCRECP-003919
Product DescriptionRecombinant Mouse Dehydrodolichyl diphosphate synthase complex subunit Nus1 (Nus1) Protein is expressed from Mammalian cell with C-terminal hFc1-tagged. It contains 140-297aa. [Accession | Q99LJ8].
Uniprot No.Q99LJ8
Gene NamesNus1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region140-297aa
SpeciesMus musculus (Mouse)
Tag InfoC-terminal hFc1-tagged
Molecular weight46.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

With DHDDS, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Both subunits contribute to enzymatic activity, i.e. condensation of multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol phosphate which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER).

Biological Context

Subcellular Location: Endoplasmic reticulum membrane (Multi-pass membrane protein)
Tissue Specificity: Highly expressed in heart, liver, kidney and pancreas
Pathway: Lipid metabolism

Product Specifications

Recombinant Mouse Dehydrodolichyl diphosphate synthase complex subunit Nus1 (Nus1), partial is a recombinant protein from Mus musculus (Mouse), expressed in Mammalian cell, covering amino acids 140-297aa, with C-terminal hFc1-tagged tag, molecular weight 46.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

With DHDDS, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery (PubMed:25066056). Both subunits contribute to enzymatic activity, i.e. condensation of multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol phosphate which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER) (By similarity). Synthesizes long-chain polyprenols, mostly of C95 and C100 chain length (By similarity). Regulates the glycosylation and stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol (By similarity). Acts as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator (By similarity)

Subcellular Location

Endoplasmic reticulum membrane (Multi-pass membrane protein)

Tissue Specificity

Highly expressed in heart, liver, kidney and pancreas

Subunit

The active dehydrodolichyl diphosphate synthase complex is a heterotetramer composed of a dimer of heterodimer of DHDDS and NUS1. Interacts with NPC2

Pathway

Lipid metabolism

Gene: Nus1  |  Organism: Mus musculus  |  Synonyms: Nogo-B receptor; Nuclear undecaprenyl pyrophosphate synthase 1 homolog

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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