Recombinant Mouse Gasdermin-A3 (Gsdma3), partial, Mus musculus (Mouse), E.coli, N-terminal 6xHis-tagged, 26.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Gasdermin-A3 (Gsdma3), partial

Recombinant Mouse Gasdermin-A3 (Gsdma3), partial — Pore-forming protein that causes membrane permeabilization and pyroptosis. Purity >90%.

SKU: BCRECP-002927 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-002927
Product DescriptionRecombinant Mouse Gasdermin-A3 (Gsdma3) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 263-464aa. [Accession | Q5Y4Y6].
Uniprot No.Q5Y4Y6
Gene NamesGsdma3
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region263-464aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged
Molecular weight26.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Pore-forming protein that causes membrane permeabilization and pyroptosis. Released upon cleavage by some bacterial effector protein, and binds to membrane inner leaflet lipids. Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the release of mature interleukin-1 (IL1B and IL18) and triggering pyroptosis.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein); Mitochondrion membrane (Multi-pass membrane protein)
Tissue Specificity: Highest levels in skin with weak expression in placenta and testis. Not detected in the gastrointestinal tract. In skin, expressed in postnatal hair follicles and epidermis as well as sebaceous gland basal cells

Product Specifications

Recombinant Mouse Gasdermin-A3 (Gsdma3), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 263-464aa, with N-terminal 6xHis-tagged tag, molecular weight 26.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Pore-forming protein that causes membrane permeabilization and pyroptosis (PubMed:26375003, PubMed:27281216, PubMed:35545613). Released upon cleavage by some bacterial effector protein, and binds to membrane inner leaflet lipids (By similarity). Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the release of mature interleukin-1 (IL1B and IL18) and triggering pyroptosis (PubMed:27281216, PubMed:33883744, PubMed:35545613). Binds to membrane inner leaflet lipids, including bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to monophosphorylated phosphatidylinositols (PubMed:27281216). Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity (PubMed:27281216, PubMed:29695864). Plays a role in the transition from catagen to telogen at the end of hair follicle morphogenesis, possibly by regulating hair follicle stem cell niche maintenance (PubMed:15475261, PubMed:15737203, PubMed:17572385, PubMed:22155111, PubMed:32302611). Also required for mammary gland development (PubMed:28168650)

Subcellular Location

Cell membrane (Multi-pass membrane protein); Mitochondrion membrane (Multi-pass membrane protein)

Tissue Specificity

Highest levels in skin with weak expression in placenta and testis. Not detected in the gastrointestinal tract. In skin, expressed in postnatal hair follicles and epidermis as well as sebaceous gland basal cells

Subunit

Homooligomer; homooligomeric ring-shaped pore complex containing 18-36 subunits when inserted in the membrane

Gene: Gsdma3  |  Organism: Mus musculus  |  Synonyms: Gasdermin-3
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top