Recombinant Mouse Interleukin-22 protein (Il22), Mus musculus (Mouse), E.Coli, Tag-Free, 16.6 kDa, purity >>96% as determined by SDS-PAGE.
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Recombinant Mouse Interleukin-22 protein (Il22)

Recombinant Mouse Interleukin-22 protein (Il22) — Cytokine that plays a critical role in modulating tissue responses during inflammation. Purity >96%.

SKU: BCACP-000724 Categories: ,
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Product SKUBCACP-000724
Product DescriptionRecombinant Mouse Interleukin-22 protein (Il22) protein is expressed from E.Coli with Tag-Free. It contains 34-179aa. [Accession | Q9JJY9].
Uniprot No.Q9JJY9
Gene NamesIl22
Purity>96% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region34-179aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight16.6 kDa
ActivityFully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 25 ng/ml, corresponding to a specific activity of >4.0x104 IU/mg.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesIl22; Il22a; Iltif; IltifaInterleukin-22; IL-22; IL-10-related T-cell-derived-inducible factor; IL-TIF; IL-TIF alpha; Interleukin-22a; IL-22a

Function

Cytokine that plays a critical role in modulating tissue responses during inflammation. Plays an essential role in the regeneration of epithelial cells to maintain barrier function after injury and for the prevention of further tissue damage. Unlike most of the cytokines, has no effect on immune cells.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Mouse Interleukin-22 protein (Il22) is a recombinant protein from Mus musculus (Mouse), expressed in E.Coli, covering amino acids 34-179aa, with Tag-Free tag, molecular weight 16.6 kDa, purity >96% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Cytokine that plays a critical role in modulating tissue responses during inflammation (PubMed:33852830, PubMed:35525330). Plays an essential role in the regeneration of epithelial cells to maintain barrier function after injury and for the prevention of further tissue damage (PubMed:33912578). Unlike most of the cytokines, has no effect on immune cells. Signals through a heterodimeric receptor composed of two subunits, the specific receptor IL22RA1 which is present on non-immune cells in many organs and the shared subunit IL10RB. Ligation of IL22RA1 with IL22 induces activation of the tyrosine kinases JAK1 and TYK2, which in turn activates STAT3. In turn, promotes cell survival and proliferation through STAT3, ERK1/2 and PI3K/AKT pathways. Promotes phosphorylation of GSK3B at 'Ser-9' and CTTN (PubMed:24742671). Promotes epithelial cell spreading (PubMed:24742671)

Subcellular Location

Secreted

Gene: Il22  |  Organism: Mus musculus  |  Synonyms: IL-10-related T-cell-derived-inducible factor; IL-TIF alpha; Interleukin-22a
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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