Recombinant Rabies virus Glycoprotein (G), partial, Rabies virus (strain ERA) (RABV), E.coli, N-terminal 6xHis-tagged, 55.0kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Rabies virus Glycoprotein (G), partial

Recombinant Rabies virus Glycoprotein (G), partial — Attaches the virus to host cellular receptor, inducing endocytosis of the virion by using different host proteins including TFRC, GRM2 and ITGB1. Purity >90%.

SKU: BCRECP-002804 Categories: ,
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Product Specifications

Product SkuBCRECP-002804
Product DescriptionRecombinant Rabies virus Glycoprotein (G) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 20-459aa. [Accession | P03524].
Uniprot No.P03524
Gene NamesG
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region20-459aa
SpeciesRabies virus (strain ERA) (RABV)
Tag InfoN-terminal 6xHis-tagged
Molecular weight55.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Attaches the virus to host cellular receptor, inducing endocytosis of the virion by using different host proteins including TFRC, GRM2 and ITGB1. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. There is convincing in vitro evidence that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and thereby facilitate rabies virus entry into cells.

Biological Context

Subcellular Location: Virion membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Rabies virus Glycoprotein (G), partial is a recombinant protein from Rabies virus (strain ERA) (RABV), expressed in E.coli, covering amino acids 20-459aa, with N-terminal 6xHis-tagged tag, molecular weight 55.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Attaches the virus to host cellular receptor, inducing endocytosis of the virion by using different host proteins including TFRC, GRM2 and ITGB1 (By similarity). In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. There is convincing in vitro evidence that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and thereby facilitate rabies virus entry into cells (By similarity)

Subcellular Location

Virion membrane (Single-pass type I membrane protein)

Subunit

Homotrimer (By similarity). Interacts with matrix protein (By similarity). Interacts with host TRFC. Interacts with host BST2; this interaction inhibits viral budding by tethering new virions to the cell surface. Interacts with ITGB1. Interacts with host GRM2 (By similarity)

Gene: G  |  Organism: Rabies virus (strain ERA)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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