Recombinant Vesicular stomatitis Indiana virus Glycoprotein G (G), partial, Vesicular stomatitis Indiana virus (strain Mudd-Summers) (VSIV), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 54.8kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Vesicular stomatitis Indiana virus Glycoprotein G (G), partial

Recombinant Vesicular stomatitis Indiana virus Glycoprotein G (G), partial — Attaches the virus to host LDL receptors, inducing clathrin-dependent endocytosis of the virion. Purity >85%.

SKU: BCRECP-002802 Categories: ,
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Product Specifications

Product SkuBCRECP-002802
Product DescriptionRecombinant Vesicular stomatitis Indiana virus Glycoprotein G (G) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-423aa. [Accession | P0C2X0].
Uniprot No.P0C2X0
Gene NamesG
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-423aa
SpeciesVesicular stomatitis Indiana virus (strain Mudd-Summers) (VSIV)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight54.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Attaches the virus to host LDL receptors, inducing clathrin-dependent endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and endosomal membrane.

Biological Context

Subcellular Location: Virion membrane (Single-pass type I membrane protein); Host membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Vesicular stomatitis Indiana virus Glycoprotein G (G), partial is a recombinant protein from Vesicular stomatitis Indiana virus (strain Mudd-Summers) (VSIV), expressed in E.coli, covering amino acids 1-423aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 54.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Attaches the virus to host LDL receptors, inducing clathrin-dependent endocytosis of the virion (PubMed:29531262). In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and endosomal membrane (PubMed:16840692, PubMed:17289996)

Subcellular Location

Virion membrane (Single-pass type I membrane protein); Host membrane (Single-pass type I membrane protein)

Subunit

Homotrimer (PubMed:16840692, PubMed:17289996). Interacts with host LDL at target cell surface (via CR2 and CR3 domains) (PubMed:29531262)

Gene: G  |  Organism: Vesicular stomatitis Indiana virus (strain Mudd-Summers)

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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