Recombinant Rotavirus A Outer capsid glycoprotein VP7, Rotavirus A (strain RVA/Cow/Canada/C486/1977/G6P6[1]) (RV-A), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 36.0kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Rotavirus A Outer capsid glycoprotein VP7

Recombinant Rotavirus A Outer capsid glycoprotein VP7 — Calcium-binding protein that interacts with rotavirus cell receptors once the initial attachment by VP4 has been achieved. Purity >85%.

SKU: BCRECP-003708 Categories: , ,
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Product Specifications

Product SkuBCRECP-003708
Product DescriptionRecombinant Rotavirus A Outer capsid glycoprotein VP7 Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 34-309aa. [Accession | A8D8S8].
Uniprot No.A8D8S8
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region34-309aa
SpeciesRotavirus A (strain RVA/Cow/Canada/C486/1977/G6P6[1]) (RV-A)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight36.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Calcium-binding protein that interacts with rotavirus cell receptors once the initial attachment by VP4 has been achieved. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. Following entry into the host cell, low intracellular or intravesicular Ca(2+) concentration probably causes the calcium-stabilized VP7 trimers to dissociate from the virion.

Biological Context

Subcellular Location: Virion; Host endoplasmic reticulum lumen

Product Specifications

Recombinant Rotavirus A Outer capsid glycoprotein VP7 is a recombinant protein from Rotavirus A (strain RVA/Cow/Canada/C486/1977/G6P6[1]) (RV-A), expressed in E.coli, covering amino acids 34-309aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 36.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Calcium-binding protein that interacts with rotavirus cell receptors once the initial attachment by VP4 has been achieved. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. Following entry into the host cell, low intracellular or intravesicular Ca(2+) concentration probably causes the calcium-stabilized VP7 trimers to dissociate from the virion. This step is probably necessary for the membrane-disrupting entry step and the release of VP4, which is locked onto the virion by VP7

Subcellular Location

Virion; Host endoplasmic reticulum lumen

Subunit

Homotrimer; disulfide-linked. 2 Ca(2+) ions bound at each subunit interface in the trimer hold the trimer together. Interacts with the intermediate capsid protein VP6. Interacts with the outer capsid protein VP5*

Organism: Rotavirus A (strain RVA/Cow/Canada/C486/1977/G6P6[1])

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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