Recombinant Sparus aurata Fibroblast growth factor (fgf2) (Active) — recombinant protein from Sparus aurata (Gilthead sea bream), expressed in E.coli. Purity >95%.
| Product SKU | BCACP-001117 |
| Product Description | Recombinant Gilthead sea bream Fibroblast growth factor (fgf2) protein is expressed from E.coli with Tag free. It contains 1-155aa. [Accession | XP_030252529.1 A0A671VAT8]. |
| Uniprot No. | XP_030252529.1 A0A671VAT8 |
| Gene Names | bFGF/FGF-2 |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 1-155aa |
| Species | Sparus aurata (Gilthead sea bream) |
| Tag Info | Tag free |
| Molecular weight | 17.1 kDa |
| Activity | Measured in a cell proliferation assay using B16-F1 cells. Recombinant Bovine Fetuin A stimulates adhesion of B16-F1 cells. The ED50 for this effect is ≤100 μg/mL.Optimal concentration depends on cell type as well as the application or research objectives. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB |
Recombinant Sparus aurata Fibroblast growth factor (fgf2) (Active) is a recombinant protein from Sparus aurata (Gilthead sea bream), expressed in E.coli, covering amino acids 1-155aa, with Tag free tag, molecular weight 17.1 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications. Explore more Growth Factor proteins →
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
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Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.
Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.
Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.
