Recombinant Mouse Urokinase-type plasminogen activator (Plau) (Active), Mus musculus (Mouse), Mammalian cell, C-terminal 10xHis-tagged, 47.4 kDa, purity >95% as determined by SDS-PAGE.
, ,

Recombinant Mouse Urokinase-type plasminogen activator (Plau) (Active)

Recombinant Mouse Urokinase-type plasminogen activator (Plau) (Active) — Serine protease that cleaves the inactive precursor plasminogen at a specific Arg-Val peptide bond, converting it into active plasmin. Purity >95%.

SKU: BCACP-000507 Categories: , ,
Online Inquiry →
Product SKUBCACP-000507
Product DescriptionRecombinant Mouse Urokinase-type plasminogen activator (Plau) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 21-433aa. [Accession | P06869].
Uniprot No.P06869
Gene NamesPlau
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region21-433aa
SpeciesMus musculus (Mouse)
Tag InfoC-terminal 10xHis-tagged
Molecular weight47.4 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human CCR1 at 10 μg/mL can bind Anti-CCR1 recombinant antibody. The EC50 is 9.690-10.94 ng/mL.The VLPs is negative control.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesUrokinase-type plasminogen activator; U-plasminogen activator; uPA; EC 3.4.21.73 [Cleaved into: Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A; Urokinase-type plasminogen activator chain B]

Function

Serine protease that cleaves the inactive precursor plasminogen at a specific Arg-Val peptide bond, converting it into active plasmin. Secreted as an inactive zymogen, it is recruited and activated at the cell surface through interaction with the urokinase plasminogen activator receptor (uPAR). Cell-surface activation enables localized, plasmin-dependent pericellular proteolysis, regulating extracellular matrix degradation in cell migration and tissue remodeling.

Product Specifications

Recombinant Mouse Urokinase-type plasminogen activator (Plau) (Active) is a recombinant protein, expressed in Mammalian cell, covering amino acids 21-433aa, with C-terminal 10xHis-tagged tag, molecular weight 47.4 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Serine protease that cleaves the inactive precursor plasminogen at a specific Arg-Val peptide bond, converting it into active plasmin (PubMed:28611361, PubMed:30707839). Secreted as an inactive zymogen, it is recruited and activated at the cell surface through interaction with the urokinase plasminogen activator receptor (uPAR). Cell-surface activation enables localized, plasmin-dependent pericellular proteolysis, regulating extracellular matrix degradation in cell migration and tissue remodeling (By similarity). Also contributes to fibrinolysis and blood clot clearance by promoting plasmin generation (PubMed:8650190)

Gene: Plau  |  Organism: Mus musculus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top