Recombinant Mouse Interleukin-1 beta protein (Il1b), Mus musculus (Mouse), E.Coli, Tag-Free, 17.5 kDa, purity >>96% as determined by SDS-PAGE.
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Recombinant Mouse Interleukin-1 beta protein (Il1b)

Recombinant Mouse Interleukin-1 beta protein (Il1b) — Potent pro-inflammatory cytokine. Purity >96%.

SKU: BCACP-000709 Categories: ,
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Product SKUBCACP-000709
Product DescriptionRecombinant Mouse Interleukin-1 beta protein (Il1b) protein is expressed from E.Coli with Tag-Free. It contains 118-269aa. [Accession | P10749].
Uniprot No.P10749
Gene NamesIl1b
Purity>96% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region118-269aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight17.5 kDa
ActivityFully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of >2x107 IU/mg.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesIl1bInterleukin-1 beta; IL-1 beta

Function

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.

Biological Context

Subcellular Location: Cytoplasm, cytosol; Secreted; Lysosome; Secreted, extracellular exosome
Tissue Specificity: Expressed in activated macrophages (at protein level)

Product Specifications

Recombinant Mouse Interleukin-1 beta protein (Il1b) is a recombinant protein from Mus musculus (Mouse), expressed in E.Coli, covering amino acids 118-269aa, with Tag-Free tag, molecular weight 17.5 kDa, purity >96% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore

Subcellular Location

Cytoplasm, cytosol; Secreted; Lysosome; Secreted, extracellular exosome

Tissue Specificity

Expressed in activated macrophages (at protein level)

Subunit

Monomer. Interacts with MEFV. Interacts with integrins ITGAV:ITGBV and ITGA5:ITGB1; integrin-binding is required for IL1B signaling. Interacts with cargo receptor TMED10; the interaction is direct and is required for the secretion of IL1B mature form (By similarity). Interacts with HSP90AB1; the interaction facilitates cargo translocation into the ERGIC (By similarity). Interacts with HSP90B1; the interaction facilitates cargo translocation into the ERGIC (By similarity)

Gene: Il1b  |  Organism: Mus musculus
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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