Recombinant Klebsiella pneumoniae Outer membrane porin C (ompC), Klebsiella pneumoniae, E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 45.0kDa, purity >85% as determined by SDS-PAGE.

Recombinant Klebsiella pneumoniae Outer membrane porin C (ompC)

Recombinant Klebsiella pneumoniae Outer membrane porin C (ompC) — Forms pores that allow passive diffusion of small molecules across the outer membrane. Purity >85%.

SKU: BCRECP-003947 Category:
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Product Specifications

Product SkuBCRECP-003947
Product DescriptionRecombinant Klebsiella pneumoniae Outer membrane porin C (ompC) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 22-363aa. [Accession | Q48473].
Uniprot No.Q48473
Gene NamesompC
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region22-363aa
SpeciesKlebsiella pneumoniae
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight45.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Forms pores that allow passive diffusion of small molecules across the outer membrane. In K.pneumoniae it has been shown to bind the C1Q component and activate the classical pathway of the complement system.

Biological Context

Subcellular Location: Cell outer membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Klebsiella pneumoniae Outer membrane porin C (ompC) is a recombinant protein from Klebsiella pneumoniae, expressed in E.coli, covering amino acids 22-363aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 45.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Forms pores that allow passive diffusion of small molecules across the outer membrane. In K.pneumoniae it has been shown to bind the C1Q component and activate the classical pathway of the complement system

Subcellular Location

Cell outer membrane (Multi-pass membrane protein)

Subunit

Homotrimer

Gene: ompC  |  Organism: Klebsiella pneumoniae  |  Synonyms: Outer membrane protein C; Porin OmpC; Porin ompk36

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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