Recombinant Human Hematopoietic progenitor cell antigen CD34 (CD34), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 28.9 kDa, purity >95% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.
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Recombinant Human Hematopoietic progenitor cell antigen CD34 (CD34), partial (Active)

Recombinant Human Hematopoietic progenitor cell antigen CD34 (CD34), partial (Active) — Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Purity >95%.

SKU: BCACP-000595 Categories: ,
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Product SKUBCACP-000595
Product DescriptionRecombinant Human Hematopoietic progenitor cell antigen CD34 (CD34) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 32-290aa. [Accession | P28906].
Uniprot No.P28906
Gene NamesCD34
PurityGreater than 95% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region32-290aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight28.9 kDa
ActivityThe ED50 as determined by the dose-dMPendent stimulation of the proliferation of NIH-3T3 cells is 0.3304-0.5261 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesHematopoietic progenitor cell antigen CD34; CD antigen CD34

Function

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Biological Context

Subcellular Location: Membrane (Single-pass type I membrane protein)
Tissue Specificity: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues

Product Specifications

Recombinant Human Hematopoietic progenitor cell antigen CD34 (CD34), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 32-290aa, with C-terminal 10xHis-tagged tag, molecular weight 28.9 kDa, purity Greater than 95% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins

Subcellular Location

Membrane (Single-pass type I membrane protein)

Tissue Specificity

Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues

Gene: CD34  |  Organism: Homo sapiens
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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