Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5)-VLPs (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 99.2 kDa, purity >/
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Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5)-VLPs (Active)

Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5)-VLPs (Active) — Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Purity /.

SKU: BCACP-000539 Categories: ,
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Product SKUBCACP-000539
Product DescriptionRecombinant Human Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5)-VLPs protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 22-907aa. [Accession | O75473].
Uniprot No.O75473
Gene NamesLGR5
Purity/
Expression SystemMammalian cell
Expression Region22-907aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight99.2 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human CXCR2 at 2 μg/mL can bind Anti-CXCR2 recombinant antibody . The EC50 is 0.3741-0.4671 ng/mL. The VLPs is negative control.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesLeucine-rich repeat-containing G-protein coupled receptor 5; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; LGR5; GPR49, GPR67

Function

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G protein-coupled receptors, does not activate heterotrimeric G proteins to transduce the signal.

Product Specifications

Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5)-VLPs (Active) is a recombinant protein, expressed in Mammalian cell, covering amino acids 22-907aa, with C-terminal 10xHis-tagged tag, molecular weight 99.2 kDa, purity /. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G protein-coupled receptors, does not activate heterotrimeric G proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development

Gene: LGR5  |  Organism: Homo sapiens

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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