Recombinant Human Mitotic spindle assembly checkpoint protein MAD2A (MAD2L1), Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 39.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Mitotic spindle assembly checkpoint protein MAD2A (MAD2L1)

Recombinant Human Mitotic spindle assembly checkpoint protein MAD2A (MAD2L1) — Component of the mitotic checkpoint complex (MCC), which inhibits APC/C and delays chromosome segregation until all chromosomes are properly attached to the mitotic spindle. Purity >90%.

SKU: BCRECP-001107 Category:
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Product Specifications

Product SkuBCRECP-001107
Product DescriptionRecombinant Human Mitotic spindle assembly checkpoint protein MAD2A (MAD2L1) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 2-205aa. [Accession | Q13257].
Uniprot No.Q13257
Gene NamesMAD2L1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-205aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight39.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Component of the mitotic checkpoint complex (MCC), which inhibits APC/C and delays chromosome segregation until all chromosomes are properly attached to the mitotic spindle. The MCC, which consists of CDC20, MAD2L1/MAD2, BUB1B/BUBR1 and BUB3, is a key player of the spindle assembly checkpoint. The MCC-bound APC/C complex is inactive and this delays the metaphase/anaphase transition.

Biological Context

Subcellular Location: Nucleus; Chromosome, centromere, kinetochore; Cytoplasm; Cytoplasm, cytoskeleton, spindle pole

Product Specifications

Recombinant Human Mitotic spindle assembly checkpoint protein MAD2A (MAD2L1) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 2-205aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 39.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Component of the mitotic checkpoint complex (MCC), which inhibits APC/C and delays chromosome segregation until all chromosomes are properly attached to the mitotic spindle (PubMed:10700282, PubMed:11804586, PubMed:15024386, PubMed:29162720, PubMed:27509861). The MCC, which consists of CDC20, MAD2L1/MAD2, BUB1B/BUBR1 and BUB3, is a key player of the spindle assembly checkpoint (PubMed:15020684, PubMed:27509861). The MCC-bound APC/C complex is inactive and this delays the metaphase/anaphase transition (PubMed:27509861). When the spindle assembly checkpoint is silenced, the MCC-APC/C complex recruits the E2 enzyme UBCH10 and triggers the auto-ubiquitination of CDC20 in the MCC, thereby reactivating APC/C for transition into anaphase (PubMed:27509861). In the closed conformation (C-MAD2) forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2 (PubMed:29162720)

Subcellular Location

Nucleus; Chromosome, centromere, kinetochore; Cytoplasm; Cytoplasm, cytoskeleton, spindle pole

Subunit

Monomer and homodimer (PubMed:18022367, PubMed:18318601). Component of the mitotic checkpoint complex (MCC), composed of CDC20, MAD2L1/MAD2, BUB1B/BUBR1 and BUB3; within the complex interacts with CDC20 (PubMed:29162720, PubMed:27509861). The MCC associates with CDC20-APC/C to form the CDC20-APC/C-MCC complex; within the complex BUB1B/BUBR1 interacts with both the CDC20 in MCC and the CDC20 in CDC20-APC/C, stabilizing the interaction between the two complexes (PubMed:27509861). Heterodimerizes with MAD1L1 in order to form a tetrameric MAD1L1-MAD2L1 core complex (PubMed:12006501, PubMed:12574116, PubMed:15024386, PubMed:18981471). In the closed and open conformation, interacts with MAD1L1 (PubMed:29162720). Formation of a heterotetrameric core complex containing two molecules each of MAD1L1 and of MAD2L1 promotes binding of another molecule of MAD2L1 to each MAD2L1, resulting in a heterohexamer (PubMed:12006501). Interacts with MAD2L1BP (PubMed:12456649, PubMed:18022368). Interacts with ADAM17/TACE (PubMed:10527948). Interacts with CDC20 (PubMed:10700282, PubMed:12574116, PubMed:18981471, PubMed:9637688). Dimeric MAD2L1 in the closed conformation interacts with CDC20 (PubMed:29162720). Monomeric MAD2L1 in the open conformation does not interact with CDC20 (PubMed:11804586). CDC20 competes with MAD1L1 for MAD2L1 binding (PubMed:11804586). In the closed conformation, interacts with BUB1B (PubMed:29162720). Interacts with TTK (PubMed:29162720). Interacts with TPR (PubMed:18981471). Binds to UBD (via ubiquitin-like 1 domain) during mitosis (PubMed:16495226, PubMed:25422469). Interacts with isoform 1 and isoform 2 of NEK2 (PubMed:20034488). Interacts with HSF1; this interaction occurs in mitosis (PubMed:18794143). Interacts with isoform 3 of MAD1L1; this interaction leads to the cytoplasmic sequestration of MAD2L1 (PubMed:19010891)

Gene: MAD2L1  |  Organism: Homo sapiens  |  Synonyms: Mitotic arrest deficient 2-like protein 1

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Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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