Recombinant Macaca fascicularis C-type lectin domain family 7 member A (CLEC7A), partial (Active), Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey), Mammalian cell, N-terminal 10xHis-tagged, 21.9 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Macaca fascicularis C-type lectin domain family 7 member A (CLEC7A), partial (Active)

Recombinant Macaca fascicularis C-type lectin domain family 7 member A (CLEC7A), partial (Active) — Lectin that functions as a pattern recognizing receptor (PRR) specific for beta-1,3-linked and beta-1,6-linked glucans, which constitute cell wall constituents from pathogenic bacteria and fungi.

SKU: BCACP-000664 Category:
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Product SKUBCACP-000664
Product DescriptionRecombinant Cynomolgus monkey C-type lectin domain family 7 member A (CLEC7A) protein is expressed from Mammalian cell with N-terminal 10xHis-tagged. It contains 100-281aa. [Accession | A0A2K5UXY6].
Uniprot No.A0A2K5UXY6
Gene NamesCLEC7A
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region100-281aa
SpeciesMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Tag InfoN-terminal 10xHis-tagged
Molecular weight21.9 kDa
ActivityMeasured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.300-2.60 ng/mL. The specific activity of recombinant human IL-15 is ≥1.50 ×108units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesC-type lectin domain family 7 member A; Dendritic cell-associated C-type lectin 1

Function

Lectin that functions as a pattern recognizing receptor (PRR) specific for beta-1,3-linked and beta-1,6-linked glucans, which constitute cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and activation of NF-kappa-B: upon beta-glucan binding, recruits SYK via its ITAM motif and promotes a signaling cascade that activates some CARD domain-BCL10-MALT1 (CBM) signalosomes, leading to the activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. Enhances cytokine production in macrophages and dendritic cells.

Biological Context

Subcellular Location: Cell membrane (Single-pass type II membrane protein)

Product Specifications

Recombinant Macaca fascicularis C-type lectin domain family 7 member A (CLEC7A), partial (Active) is a recombinant protein from Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey), expressed in Mammalian cell, covering amino acids 100-281aa, with N-terminal 10xHis-tagged tag, molecular weight 21.9 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Lectin that functions as a pattern recognizing receptor (PRR) specific for beta-1,3-linked and beta-1,6-linked glucans, which constitute cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and activation of NF-kappa-B: upon beta-glucan binding, recruits SYK via its ITAM motif and promotes a signaling cascade that activates some CARD domain-BCL10-MALT1 (CBM) signalosomes, leading to the activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Induces phosphorylation of SCIMP after binding beta-glucans

Subcellular Location

Cell membrane (Single-pass type II membrane protein)

Subunit

Homodimer. Interacts with SYK; participates in leukocyte activation in presence of fungal pathogens. Interacts with CD37; this interaction controls CLEC7A-mediated IL-6 production

Organism: Macaca fascicularis  |  Synonyms: Dendritic cell-associated C-type lectin 1

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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