Recombinant Mouse Sonic hedgehog protein (Shh), partial (Active), Mus musculus (Mouse), E.Coli, Tag-Free, 19.8 kDa, purity >>95% as determined by SDS-PAGE.

Recombinant Mouse Sonic hedgehog protein (Shh), partial (Active)

Recombinant Mouse Sonic hedgehog protein (Shh), partial (Active) — The dually lipidated sonic hedgehog protein N-product (ShhN) is a morphogen that activates the smoothened signaling pathway, and which is essential for a variety of patterning events during develop… Purity >95%.

SKU: BCACP-000875 Category:
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Product SKUBCACP-000875
Product DescriptionRecombinant Mouse Sonic hedgehog protein (Shh) protein is expressed from E.Coli with Tag-Free. It contains 26-198aa. [Accession | Q62226].
Uniprot No.Q62226
Gene NamesShh
Purity>95% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region26-198aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight19.8 kDa
ActivityFully biologically active when compared to standard. The binding affinity of rMuFABP1 for the synthetic ligand cis-parinaric acid has been measured by fluorescence titration. Half maximal fluorescence of 2.5 μM rMuFABP1 is achieved with approximately 5 μM cis-paranaric acid.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesShh; Hhg1; Sonic hedgehog protein; SHH; HHG-1; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; ShhNC) [Cleaved into: Sonic hedgehog protein N-product; ShhN; Shh N-terminal processed signaling domains; ShhNp; Sonic hedgehog protein 19 kDa product)]

Function

The dually lipidated sonic hedgehog protein N-product (ShhN) is a morphogen that activates the smoothened signaling pathway, and which is essential for a variety of patterning events during development. Acts by binding to the patched receptor (PTCH1 or PTCH2), relieving smoothened (SMO) inhibition by patched, activating the smoothened signaling pathway and transcription of target genes. In the absence of SHH, patched represses the constitutive signaling activity of SMO.

Biological Context

Subcellular Location: Secreted; Cell membrane (Lipid-anchor)
Tissue Specificity: Expressed in a number of embryonic tissues including the notochord, ventral neural tube, floor plate, lung bud, zone of polarizing activity and posterior distal mesenchyme of limbs. In the adult, expressed in lung and neural retina

Product Specifications

Recombinant Mouse Sonic hedgehog protein (Shh), partial (Active) is a recombinant protein from Mus musculus (Mouse), expressed in E.Coli, covering amino acids 26-198aa, with Tag-Free tag, molecular weight 19.8 kDa, purity >95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Fully biologically active when compared to standard. The binding affinity of rMuFABP1 for the synthetic ligand cis-parinaric acid has been measured by fluorescence titration. Half maximal fluorescence of 2.5 μM rMuFABP1 is achieved with approximately 5 μM cis-paranaric acid.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

The dually lipidated sonic hedgehog protein N-product (ShhN) is a morphogen that activates the smoothened signaling pathway, and which is essential for a variety of patterning events during development (PubMed:11389830, PubMed:14687547, PubMed:29622726, PubMed:7736596, PubMed:7916661). Acts by binding to the patched receptor (PTCH1 or PTCH2), relieving smoothened (SMO) inhibition by patched, activating the smoothened signaling pathway and transcription of target genes (PubMed:31657721). In the absence of SHH, patched represses the constitutive signaling activity of SMO (PubMed:31657721). ShhN is widely expressed and plays a key role in the patterning of the central nervous system and limb development (PubMed:11430830, PubMed:12679031, PubMed:15315762, PubMed:24863049). Induces ventral cell fate in the neural tube and somites (PubMed:11430830, PubMed:24863049). Involved in the patterning of the anterior-posterior axis of the developing limb bud (PubMed:15315762). Essential for axon guidance (PubMed:12679031)

Subcellular Location

Secreted; Cell membrane (Lipid-anchor)

Tissue Specificity

Expressed in a number of embryonic tissues including the notochord, ventral neural tube, floor plate, lung bud, zone of polarizing activity and posterior distal mesenchyme of limbs (PubMed:7916661). In the adult, expressed in lung and neural retina (PubMed:7916661)

Subunit

Multimer (PubMed:15075292, PubMed:22677548, PubMed:22902404, PubMed:24522195). Interacts with SCUBE2; SCUBE2 acts as a carrier to promote release of the dual-lipidated sonic hedgehog protein N-product (ShhN) from the cell surface (PubMed:22677548, PubMed:24522195). Interacts with DISP1 via its cholesterol anchor (PubMed:22677548, PubMed:22902404). Interacts with BOC and CDON; BOC and CDON form a heterodimer that acts as a coreceptor for ShhN (PubMed:18794898, PubMed:20519495). Interacts with GAS1; GAS1 acts as a coreceptor for ShhN (PubMed:33038332)

Gene: Shh  |  Organism: Mus musculus  |  Synonyms: HHG-1; Shh unprocessed N-terminal signaling and C-terminal autoprocfretinaessing domains
Key Publications

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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