Recombinant Rat Aquaporin-2 (Aqp2) Protein, Rattus norvegicus (Rat), in vitro E.coli expression system, N-terminal 10xHis-tagged, 30.4kDa, purity >85% as determined by SDS-PAGE.

Recombinant Rat Aquaporin-2 (Aqp2) Protein

Recombinant Rat Aquaporin-2 (Aqp2) Protein — Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Purity >85%.

SKU: BCRECP-00294TM Category:
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Product Specifications

Uniprot No.P34080
Gene NamesAqp2
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-271aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 10xHis-tagged
Molecular weight30.4kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Plays an essential role in renal water homeostasis. Could also be permeable to glycerol.

Biological Context

Subcellular Location: Apical cell membrane (Multi-pass membrane protein); Basolateral cell membrane (Multi-pass membrane protein); Cell membrane (Multi-pass membrane protein); Cytoplasmic vesicle membrane (Multi-pass membrane protein); Golgi apparatus, trans-Golgi network membrane (Multi-pass membrane protein)
Tissue Specificity: Detected in kidney, in cortical and the medullary collecting tubules (at protein level). Detected in kidney medulla and cortex

Product Specifications

Recombinant Rat Aquaporin-2 (Aqp2) Protein is a recombinant protein from Rattus norvegicus (Rat), expressed in in vitro E.coli expression system, covering amino acids 1-271aa, with N-terminal 10xHis-tagged tag, molecular weight 30.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Not implied unless activity data are explicitly listed on this product page, the datasheet, or the CoA.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient (PubMed:7508187, PubMed:8429910). Plays an essential role in renal water homeostasis (By similarity). Could also be permeable to glycerol (By similarity)

Subcellular Location

Apical cell membrane (Multi-pass membrane protein); Basolateral cell membrane (Multi-pass membrane protein); Cell membrane (Multi-pass membrane protein); Cytoplasmic vesicle membrane (Multi-pass membrane protein); Golgi apparatus, trans-Golgi network membrane (Multi-pass membrane protein)

Tissue Specificity

Detected in kidney, in cortical and the medullary collecting tubules (at protein level) (PubMed:8429910). Detected in kidney medulla and cortex (PubMed:7508187, PubMed:8429910)

Subunit

Homotetramer

Gene: Aqp2  |  Organism: Rattus norvegicus  |  Synonyms: ADH water channel; Aquaporin-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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